One possibility is that your weekly use of PolyQuat 60 results in measurable CC. You might try measuring it soon after an application and then a week later before another application and see if you notice any difference. It is known that chlorine will react with PolyQuat 60, but I do not know if it will show up as CC in the chlorine test. We do know that non-chlorine shock (potassium monopersulfate, KMPS) shows up as CC so it is quite possible that PolyQuat 60 in the presence of chlorine also shows up as CC.

As for the number of scoops and the CYA mixing, I suspect it won't matter very much. For the chlorine test you just need to have enough powder to develop enough color even for low levels of chlorine. The titrant will make it colorless regardless. It's just like indicator drops where color will shift -- it's only an issue if you are measuring the intensity of the color with a colorimeter as opposed to seeing a shift in the color (red to blue for CH; pink to colorless for FC; green to red for TA). The CYA test just needs mixing and then time to develop so continuing to mix or letting it sit should work either way so long as it is thoroughly mixed initially.

Richard