OK, you've got two inconsistent test results.

In post #43, you report "the black dot did not disappear". That means a CYA level of LESS THAN 40 ppm. But, in post #41, you report a CYA level of 100+, which means that your dot should have disappeared, when using the 50/50 mixture, at a level of 50 or MORE. So, the first result is that your CYA is MORE than 100 ppm, but your second result is that it's LESS than 40 ppm. One, or both, of your tests are in error.

So, please test AGAIN, with straight pool water, and tell me what you have. Please, PLEASE re-read the instructions, and make certain you are performing the test correctly.